Systems Analysis of Auxin Transport in the Arabidopsis Root Apex

被引:167
作者
Band, Leah R. [1 ]
Wells, Darren M. [1 ]
Fozard, John A. [1 ]
Ghetiu, Teodor [1 ]
French, Andrew P. [1 ]
Pound, Michael P. [1 ]
Wilson, Michael H. [1 ]
Yu, Lei [1 ]
Li, Wenda [1 ]
Hijazi, Hussein I. [1 ]
Oh, Jaesung [1 ]
Pearce, Simon P. [1 ]
Perez-Amador, Miguel A. [2 ]
Yun, Jeonga [3 ]
Kramer, Eric [4 ]
Alonso, Jose M. [3 ]
Godin, Christophe [5 ]
Vernoux, Teva [6 ]
Hodgman, T. Charlie [1 ]
Pridmore, Tony P. [1 ]
Swarup, Ranjan [1 ]
King, John R. [1 ]
Bennett, Malcolm J. [1 ]
机构
[1] Univ Nottingham, Ctr Plant Integrat Biol, Nottingham LE12 5RD, England
[2] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain
[3] N Carolina State Univ, Dept Genet, Raleigh, NC 27695 USA
[4] Bard Coll Simons Rock, Dept Phys, Great Barrington, MA 01230 USA
[5] Inst Natl Rech Informat & Automat, Ctr Cooperat Int Rech Agronom Dev, Virtual Plants Project Team, Unite Mixte Rech Ameliorat Genet Plantes Mediterr, F-34095 Montpellier, France
[6] Univ Lyon 1, Ecole Normale Super Lyon, INRA, CNRS,Lab Reprod & Dev Plantes, F-69364 Lyon, France
基金
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会; 美国国家科学基金会;
关键词
PLANT DEVELOPMENT; PERMEASE AUX1; HAIR-CELLS; GRAVITROPISM; LOCALIZATION; GRADIENT; GROWTH; BIOSYNTHESIS; PROTEINS; FAMILY;
D O I
10.1105/tpc.113.119495
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin's shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues.
引用
收藏
页码:862 / 875
页数:14
相关论文
共 57 条
[1]   Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism [J].
Abas, L ;
Benjamins, R ;
Malenica, N ;
Paciorek, T ;
Wirniewska, J ;
Moulinier-Anzola, JC ;
Sieberer, T ;
Friml, J ;
Luschnig, C .
NATURE CELL BIOLOGY, 2006, 8 (03) :249-256
[2]   Multiscale modelling of auxin transport in the plant-root elongation zone [J].
Band, L. R. ;
King, J. R. .
JOURNAL OF MATHEMATICAL BIOLOGY, 2012, 65 (04) :743-785
[3]   Root gravitropism is regulated by a transient lateral auxin gradient controlled by a tipping-point mechanism [J].
Band, Leah R. ;
Wells, Darren M. ;
Larrieu, Antoine ;
Sun, Jianyong ;
Middleton, Alistair M. ;
French, Andrew P. ;
Brunoud, Geraldine ;
Sato, Ethel Mendocilla ;
Wilson, Michael H. ;
Peret, Benjamin ;
Oliva, Marina ;
Swarup, Ranjan ;
Sairanen, Ilkka ;
Parry, Geraint ;
Ljung, Karin ;
Beeckman, Tom ;
Garibaldi, Jonathan M. ;
Estelle, Mark ;
Owen, Markus R. ;
Vissenberg, Kris ;
Hodgman, T. Charlie ;
Pridmore, Tony P. ;
King, John R. ;
Vernoux, Teva ;
Bennett, Malcolm J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (12) :4668-4673
[4]   Auxin: The looping star in plant development [J].
Benjamins, Rene ;
Scheres, Ben .
ANNUAL REVIEW OF PLANT BIOLOGY, 2008, 59 :443-465
[5]   Local, efflux-dependent auxin gradients as a common module for plant organ formation [J].
Benková, E ;
Michniewicz, M ;
Sauer, M ;
Teichmann, T ;
Seifertová, D ;
Jürgens, G ;
Friml, J .
CELL, 2003, 115 (05) :591-602
[6]   The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots [J].
Blilou, I ;
Xu, J ;
Wildwater, M ;
Willemsen, V ;
Paponov, I ;
Friml, J ;
Heidstra, R ;
Aida, M ;
Palme, K ;
Scheres, B .
NATURE, 2005, 433 (7021) :39-44
[7]   A novel sensor to map auxin response and distribution at high spatio-temporal resolution [J].
Brunoud, Geraldine ;
Wells, Darren M. ;
Oliva, Marina ;
Larrieu, Antoine ;
Mirabet, Vincent ;
Burrow, Amy H. ;
Beeckman, Tom ;
Kepinski, Stefan ;
Traas, Jan ;
Bennett, Malcolm J. ;
Vernoux, Teva .
NATURE, 2012, 482 (7383) :103-U132
[8]   The F-box protein TIR1 is an auxin receptor [J].
Dharmasiri, N ;
Dharmasiri, S ;
Estelle, M .
NATURE, 2005, 435 (7041) :441-445
[9]   Changes in root cap pH are required for the gravity response of the Arabidopsis root [J].
Fasano, JM ;
Swanson, SJ ;
Blancaflor, EB ;
Dowd, PE ;
Kao, TH ;
Gilroy, S .
PLANT CELL, 2001, 13 (04) :907-921
[10]   Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis [J].
Friml, J ;
Wisniewska, J ;
Benkova, E ;
Mendgen, K ;
Palme, K .
NATURE, 2002, 415 (6873) :806-809