Cation-selective mutations in the M2 domain of the inhibitory glycine receptor channel reveal determinants of ion-charge selectivity

Ligand-gated ion channel receptors mediate neuronal inhibition or excitation depending on their ion charge selectivity An investigation into the determinants of ion charge selectivity of the anion-selective alpha1 homomeric glycine receptor (alpha1 glycine receptor [GlyR]) was undertaken using point mutations to residues lining the extra- and intracellular ends of the ion channel. Five mutant GlyRs were studied. A single substitution at the intracellular month of the channel (A-1'E GlyR) was sufficient to convert the channels to select cations over anions with P-Cl/P-Na = 0.34. This result delimits the selectivity filter and provides evidence that electrostatic interactions between permeating ions and pore residues are a critical factor in ion charge selectivity. The P-2'Delta mutant GlyR retained its anion selectivity (P-Cl/P-Na = 3.81), but it was much reduced compared with the wild-type (WT) GlyR (P-Cl/P-Na = 27.9). When the A-1'E and the P-TA mutations were combined (selectivity double mutant [SDM] GlyR), the relative cation permeability was enhanced (P-Cl/P-Na = 0.13). The SDM GlyR was also Ca2+ permeable (P-Ca/P-Na = 0.29). Neutralizing the extracellular mouth of the SDM GlyR ion channel (SDM+R19'A GlyR) produced a more Ca2+-permeable channel (P-Ca/P-Na = 0.73), without drastically altering monovalent charge selectivity (P-Cl/P-Na = 0.23). The SDM+R19'E GlyR, which introduces a negatively charged ring at the extracellular month of the channel, further enhanced Ca2+ permeability (P-Cl/P-Na = 0.92), with little effect on monovalent selectivity (P-Cl/P-Na = 0.19). Estimates of the minimum pore diameter of the A-1'E, SDM, SDM+R19'A, and SDM+R19'E GlyRs revealed that these pores are larger than the alpha1 GlyR, with the SDM-based GlyRs being comparable in diameter to the cation-selective nicotinic acetylcholine receptors. This result provides evidence that the diameter of the ion channel is also an important factor in ion charge selectivity.