Identification of Asian pear varieties by SSR analysis

被引：97

作者：

Kimura, T ^{[1
]}

Shi, YZ ^{[1
]}

Shoda, M ^{[1
]}

Kotobuki, K ^{[1
]}

Matsuta, N ^{[1
]}

Hayashi, T ^{[1
]}

Ban, Y ^{[1
]}

Yamamoto, T ^{[1
]}

机构：

[1] Natl Inst Fruit Tree Sci, Tsukuba, Ibaraki 3058605, Japan

来源：

BREEDING SCIENCE | 2002年 / 52卷 / 02期

关键词：

Pyrus spp; genetic diversity; genetic relatedness; microsatellites; simple sequence repeat;

D O I：

10.1270/jsbbs.52.115

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Sixty Asian pear accessions from 6 Pyrus species were genetically identified by 9 SSR markers with a total of 133 putative alleles. Among them, 58 varieties could be successfully differentiated except for 2 pairs of synonymous or clonal varieties. All the SSR markers produced 1 or 2 discrete amplified fragments for all the diploid accessions, whereas a triploid variety showed 3 fragments with some SSRs. The number of putative alleles ranged from 7 to 20, with an average value of 14.8. The observed heterozygosity and the power of discrimination were 0.63 and 0.91, respectively. A phenogram based on the SSR genotypes was obtained, showing 3 major groups corresponding to the Japanese, Chinese and European groups. The SSR markers were highly polymorphic and could be utilized as a reliable tool for cultivar identification in Asian pears.

引用

页码：115 / 121

页数：7

共 30 条

[1]

Bell R. L., 1990, Acta Horticulturae, P655

[2]

BELL RL, 1986, HORTSCIENCE, V21, P62

[3]

Bell RL, 1996, FRUIT BREEDING, P441

[4] Inheritance and linkage of isozyme loci in pear (Pyrus communis L) [J].

Chevreau, E ;

Leuliette, S ;

Gallet, M .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (3-4) :498-506

[5] RANDOM AMPLIFIED HYBRIDIZATION MICROSATELLITES (RAHM) - ISOLATION OF A NEW CLASS OF MICROSATELLITE-CONTAINING DNA CLONES [J].

CIFARELLI, RA ;

GALLITELLI, M ;

CELLINI, F .

NUCLEIC ACIDS RESEARCH, 1995, 23 (18) :3802-3803

[6] AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L) Batsch]:: isolation, characterisation and cross-species amplification in Prunus [J].

Cipriani, G ;

Lot, G ;

Huang, WG ;

Marrazzo, MT ;

Peterlunger, E ;

Testolin, R .

THEORETICAL AND APPLIED GENETICS, 1999, 99 (1-2) :65-72

[7] Polymorphic DNA markers in black cherry (Prunus serotina) are identified using sequences from sweet cherry, peach, and sour cherry [J].

Downey, SL ;

Iezzoni, AF .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2000, 125 (01) :76-80

[8] Single locus microsatellites isolated using 5' anchored PCR [J].

Fisher, PJ ;

Gardner, RC ;

Richardson, TE .

NUCLEIC ACIDS RESEARCH, 1996, 24 (21) :4369-4371

[9] Simple sequence repeats for the genetic analysis of apple [J].

Gianfranceschi, L ;

Seglias, N ;

Tarchini, R ;

Komjanc, M ;

Gessler, C .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1069-1076

[10] Microsatellites in Malus X domestica (apple): Abundance, polymorphism and cultivar identification [J].

Guilford, P ;

Prakash, S ;

Zhu, JM ;

Rikkerink, E ;

Gardiner, S ;

Bassett, H ;

Forster, R .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (02) :249-254

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