Automation of NMR measurements and data evaluation for systematically screening interactions of small molecules with target proteins

被引：45

作者：

Ross, A ^{[1
]}

Schlotterbeck, G ^{[1
]}

Klaus, W ^{[1
]}

Senn, H ^{[1
]}

机构：

[1] Hoffmann LaRoche AG, Dept Biostruct Res PRPCS, CH-4070 Basel, Switzerland

来源：

JOURNAL OF BIOMOLECULAR NMR | 2000年 / 16卷 / 02期

关键词：

flow injection; protein; screening;

D O I：

10.1023/A:1008394910612

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this technical note we describe the setup and application of automated sample preparation and usage of flow-through NMR equipment for the characterization of ligand binding on proteins. In addition, we focus on the perspectives of automated analysis of 2D HSQC spectra to identify changes in patterns indicative for ligand binding or changes of sample conditions. In this context we discuss a combination of statistical and non-statistical data analysis.

引用

页码：139 / 146

页数：8

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