Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract

被引：305

作者：

Gomez-Pinilla, Pedro J. ^{[1
,2
]}

Gibbons, Simon J. ^{[1
,2
]}

Bardsley, Michael R. ^{[1
,2
]}

Lorincz, Andrea ^{[1
,2
]}

Pozo, Maria J. ^{[3
,4
]}

Pasricha, Pankaj J. ^{[6
]}

Van de Rijn, Matt ^{[7
]}

West, Robert B. ^{[7
]}

Sarr, Michael G. ^{[5
]}

Kendrick, Michael L. ^{[5
]}

Cima, Robert R. ^{[5
]}

Dozois, Eric J. ^{[5
]}

Larson, David W. ^{[5
]}

Ordog, Tamas ^{[1
,2
]}

Farrugia, Gianrico ^{[1
,2
]}

机构：

[1] Mayo Clin, Enter Neurosci Program, Miles & Shirley Fiterman Ctr Digest Dis, Rochester, MN 55905 USA

[2] Mayo Clin, Dept Physiol & Biomed Engn, Rochester, MN 55905 USA

[3] Univ Extremadura, Sch Nursing, Dept Physiol, Caceres, Spain

[4] RETICEF, Caceres, Spain

[5] Mayo Clin, Dept Surg, Rochester, MN 55905 USA

[6] Stanford Univ, Dept Med, Sch Med, Div Gastroenterol, Stanford, CA 94305 USA

[7] Stanford Univ, Med Ctr, Dept Pathol, Stanford, CA 94305 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2009年 / 296卷 / 06期

关键词：

Kit; mast cells; chloride channels; gastrointestinal motility; immunofluorescence; SLOW-TRANSIT CONSTIPATION; MURINE SMALL-INTESTINE; C-KIT; PACEMAKER ACTIVITY; CIRCULAR MUSCLE; DIABETIC GASTROPARESIS; FUNCTIONAL CLASSES; STROMAL TUMORS; GENE-PRODUCTS; EXPRESSION;

D O I：

10.1152/ajpgi.00074.2009

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, Van de Rijn M, West RB, Sarr MG, Kendrick ML, Cima RR, Dozois EJ, Larson DW, Ordog T, Farrugia G. Ano1 is a selective marker of interstitial cells of Cajal in the human and mouse gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 296: G1370-G1381, 2009. First published April 16, 2009; doi:10.1152/ajpgi.00074.2009.-Populations of interstitial cells of Cajal (ICC) are altered in several gastrointestinal neuromuscular disorders. ICC are identified typically by ultrastructure and expression of Kit (CD117), a protein that is also expressed on mast cells. No other molecular marker currently exists to independently identify ICC. The expression of ANO1 (DOG1, TMEM16A), a Ca2+-activated Cl- channel, in gastrointestinal stromal tumors suggests it may be useful as an ICC marker. The aims of this study were therefore to determine the distribution of Ano1 immunoreactivity compared with Kit and to establish whether Ano1 is a reliable marker for human and mouse ICC. Expression of Ano1 in human and mouse stomach, small intestine, and colon was investigated by immunofluorescence labeling using antibodies to Ano1 alone and in combination with antibodies to Kit. Colocalization of immunoreactivity was demonstrated by epifluorescence and confocal microscopy. In the muscularis propria, Ano1 immunoreactivity was restricted to cells with the morphology and distribution of ICC. All Ano1-positive cells in the muscularis propria were also Kit positive. Kit-expressing mast cells were not Ano1 positive. Some non-ICC in the mucosa and submucosa of human tissues were Ano1 positive but Kit negative. A few (3.2%) Ano1-positive cells in the human gastric muscularis propria were labeled weakly for Kit. Ano1 labels all classes of ICC and represents a highly specific marker for studying the distribution of ICC in mouse and human tissues with an advantage over Kit since it does not label mast cells.

引用

页码：G1370 / G1381

页数：12

共 41 条

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