Single-cell gene expression profiling

被引：383

作者：

Levsky, JM ^{[1
]}

Shenoy, SM ^{[1
]}

Pezo, RC ^{[1
]}

Singer, RH ^{[1
]}

机构：

[1] Yeshiva Univ Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 USA

来源：

SCIENCE | 2002年 / 297卷 / 5582期

关键词：

Bioassay - Cell culture - Fluorescence - Genetic engineering - Microscopic examination - Substrates;

D O I：

10.1126/science.1072241

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A key goal of biology is to relate the expression of specific genes to a particular cellular phenotype. However, current assays for gene expression destroy the structural context. By combining advances in computational fluorescence microscopy with multiplex probe design, we devised technology in which the expression of many genes can be visualized simultaneously inside single cells with high spatial and temporal resolution. Analysis of 11 genes in serum-stimulated cultured cells revealed unique patterns of gene expression within individual cells. Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: "cellular genomics."

引用

页码：836 / 840

页数：6

共 20 条

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