Generation of retinal cells from mouse and human induced pluripotent stem cells

被引：331

作者：

Hirami, Yasuhiko ^{[1
,2
]}

Osakada, Fumitaka ^{[1
]}

Takahashi, Kazutoshi ^{[3
,4
]}

Okita, Keisuke ^{[3
,4
]}

Yamanaka, Shinya ^{[3
,4
]}

Ikeda, Hanako ^{[2
]}

Yoshimura, Nagahisa ^{[2
]}

Takahashi, Masayo ^{[1
]}

机构：

[1] RIKEN, Ctr Dev Biol, Lab Retinal Regenerat, Chuo Ku, Kobe, Hyogo 6500047, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Ophthalmol, Kyoto 6068501, Japan

[3] Kyoto Univ, Inst Frontier Med Sci, Dept Stem Cell Biol, Kyoto 6068501, Japan

[4] Kyoto Univ, Inst Integrated Cell Mat Sci, Ctr iPS Cell Res & Applicat, Kyoto 6068501, Japan

来源：

NEUROSCIENCE LETTERS | 2009年 / 458卷 / 03期

关键词：

Differentiation; Induced pluripotent stem cell; Photoreceptor; Retinal pigment epithelium; HOMEOBOX GENE; MACULAR DEGENERATION; HUMAN FIBROBLASTS; EYE DEVELOPMENT; DIFFERENTIATION; PRECURSORS; TRANSPLANTATION; PHOTORECEPTORS; EXPRESSION; CRX;

D O I：

10.1016/j.neulet.2009.04.035

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We previously reported a technique for generating retinal pigment epithelia (RPE) and putative photoreceptors from embryonic stem (ES) cells. Here we tested whether our procedure can promote retinal differentiation of mouse and human induced pluripotent stem cells (iPSCs). Treating iPSCs with Wnt and Nodal antagonists in suspension culture induced expression of markers of retinal progenitor cells and generated RIDE cells. Subsequently, treatment with retinoic acid and taurine generated cells positive for photoreceptor markers in all but one human cell lines. We propose that iPSCs can be induced to differentiate into retinal cells which have a possibility to be used as patient-specific donor cells for transplantation therapies. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：126 / 131

页数：6

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