Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

被引：15

作者：

Horsey, I ^{[1
]}

Krishnan-Ghosh, Y ^{[1
]}

Balasubramanian, S ^{[1
]}

机构：

[1] Univ Cambridge, Chem Labs, Cambridge CB2 1EW, England

来源：

CHEMICAL COMMUNICATIONS | 2002年 / 17期

关键词：

D O I：

10.1039/b206054j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 degreesC increase in T-m of the complex.

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页码：1950 / 1951

页数：2

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