A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein

被引：39

作者：

Boudreau, E

Turmel, M

GoldschmidtClermont, M

Rochaix, JD

Sivan, S

Michaels, A

Leu, S

机构：

[1] BEN GURION UNIV NEGEV, DORIS & BERTIE BLACK CTR BIOENERGET LIFE SCI, IL-84105 BEER SHEVA, ISRAEL

[2] UNIV LAVAL, FAC SCI & GENIE, DEPT BIOCHIM, LAVAL, PQ G1K 7P4, CANADA

[3] UNIV GENEVA, DEPT BIOL MOL, CH-1211 GENEVA 4, SWITZERLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1997年 / 253卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

green algae; Chlamydomonas reinhardtii; chloroplast genome; gene inactivation; transmembrane protein;

D O I：

10.1007/s004380050368

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.

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收藏

页码：649 / 653

页数：5

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