TRANSGENIC EXPRESSION OF AMINOGLYCOSIDE ADENINE TRANSFERASE IN THE CHLOROPLAST - A SELECTABLE MARKER FOR SITE-DIRECTED TRANSFORMATION OF CHLAMYDOMONAS

被引：363

作者：

GOLDSCHMIDTCLERMONT, M ^{[1
]}

机构：

[1] UNIV GENEVA,DEPT MOLEC BIOL,CH-1211 GENEVA 4,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 15期

关键词：

D O I：

10.1093/nar/19.15.4083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression vectors for Chlamydomonas reinhardtii chloroplast transformation have been constructed with transcription and translation signals from chloroplast genes. The bacterial aadA sequence, coding for aminoglycoside 3" adenyl transferase, was inserted in these vectors and introduced into the C. reinhardtii chloroplast by particle gun transformation. The stable transgenic expression of this foreign protein in the chloroplast confers spectinomycin and streptomycin resistance to the transformed cells. This new marker can be used as a reporter of gene expression, and as a portable selectable cassette for chloroplast reverse genetics. Targetted gene disruption mutants of loci required for photosynthesis, tscA and psaC, were thus obtained. A gene disruption of an unidentified open reading frame, ORF472, remained heteroplasmic, suggesting that it has a vital function.

引用

页码：4083 / 4089

页数：7

共 33 条

[1] STUDIES ON CHLAMYDOMONAS CHLOROPLAST TRANSFORMATION - FOREIGN DNA CAN BE STABLY MAINTAINED IN THE CHROMOSOME [J].

BLOWERS, AD ;

BOGORAD, L ;

SHARK, KB ;

SANFORD, JC .

PLANT CELL, 1989, 1 (01) :123-132

[2]

BLOWERS AD, 1990, PLANT CELL, V2, P1059

[3] CHLOROPLAST TRANSFORMATION IN CHLAMYDOMONAS WITH HIGH-VELOCITY MICROPROJECTILES [J].

BOYNTON, JE ;

GILLHAM, NW ;

HARRIS, EH ;

HOSLER, JP ;

JOHNSON, AM ;

JONES, AR ;

RANDOLPHANDERSON, BL ;

ROBERTSON, D ;

KLEIN, TM ;

SHARK, KB ;

SANFORD, JC .

SCIENCE, 1988, 240 (4858) :1534-1538

[4]

BOYNTON JE, 1990, CURRENT RES PHOTOSYN, V3, P509

[5] MUTANT PHENOTYPES SUPPORT A TRANS-SPLICING MECHANISM FOR THE EXPRESSION OF THE TRIPARTITE PSAA GENE IN THE C-REINHARDTII CHLOROPLAST [J].

CHOQUET, Y ;

GOLDSCHMIDTCLERMONT, M ;

GIRARDBASCOU, J ;

KUCK, U ;

BENNOUN, P ;

ROCHAIX, JD .

CELL, 1988, 52 (06) :903-913

[6] TRANSIENT FOREIGN GENE-EXPRESSION IN CHLOROPLASTS OF CULTURED TOBACCO CELLS AFTER BIOLISTIC DELIVERY OF CHLOROPLAST VECTORS [J].

DANIELL, H ;

VIVEKANANDA, J ;

NIELSEN, BL ;

YE, GN ;

TEWARI, KK ;

SANFORD, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :88-92

[7] THE ARGININOSUCCINATE LYASE GENE OF CHLAMYDOMONAS-REINHARDTII - AN IMPORTANT TOOL FOR NUCLEAR TRANSFORMATION AND FOR CORRELATING THE GENETIC AND MOLECULAR MAPS OF THE ARG7 LOCUS [J].

DEBUCHY, R ;

PURTON, S ;

ROCHAIX, JD .

EMBO JOURNAL, 1989, 8 (10) :2803-2809

[8] SEQUENCE OF THE CHLOROPLAST DNA REGION OF CHLAMYDOMONAS-REINHARDII CONTAINING THE GENE OF THE LARGE SUBUNIT OF RIBULOSE BISPHOSPHATE CARBOXYLASE AND PARTS OF ITS FLANKING GENES [J].

DRON, M ;

RAHIRE, M ;

ROCHAIX, JD .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 162 (04) :775-793

[9] A SMALL CHLOROPLAST RNA MAY BE REQUIRED FOR TRANSSPLICING IN CHLAMYDOMONAS-REINHARDTII [J].

GOLDSCHMIDTCLERMONT, M ;

CHOQUET, Y ;

GIRARDBASCOU, J ;

MICHEL, F ;

SCHIRMERRAHIRE, M ;

ROCHAIX, JD .

CELL, 1991, 65 (01) :135-143

[10] TRANSSPLICING MUTANTS OF CHLAMYDOMONAS-REINHARDTII [J].

GOLDSCHMIDTCLERMONT, M ;

GIRARDBASCOU, J ;

CHOQUET, Y ;

ROCHAIX, JD .

MOLECULAR & GENERAL GENETICS, 1990, 223 (03) :417-425

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