The influence of solvent viscosity on the fluorescence decay and time-resolved anisotropy of green fluorescent protein

被引：56

作者：

Suhling, K

Davis, DM

Phillips, D

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

来源：

JOURNAL OF FLUORESCENCE | 2002年 / 12卷 / 01期

关键词：

GFP fluorescence lifetime; anisotropy; TCSPC; viscosity;

D O I：

10.1023/A:1015323606154

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This report describes fluorescence decay and time-resolved anisotropy studies of green fluorescent protein (GFP) in various environments. The addition of glucose and fructose, NaCl, or polyethylene glycol changes the viscosity of the medium surrounding the GFP. Both the time-resolved anisotropy and the fluorescence decay of GFP are measured and it is shown that only the time-resolved anisotropy of GFP is affected by the viscosity, but not its fluorescence decay.

引用

页码：91 / 95

页数：5

共 23 条

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