Molecular and biochemical survey of acaricide resistance mechanisms in larvae from Mexican strains of the southern cattle tick, Boophilus microplus

被引:113
作者
Jamroz, RC [1 ]
Guerrero, FD [1 ]
Pruett, JH [1 ]
Oehler, DD [1 ]
Miller, RJ [1 ]
机构
[1] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA
关键词
acaricide resistance; carboxylesterases; degenerate RT-PCR; organophosphates; pyrethroids; ticks;
D O I
10.1016/S0022-1910(99)00157-2
中图分类号
Q96 [昆虫学];
学科分类号
摘要
We examined the larvae of several organophosphate and pyrethroid-resistant Mexican strains of Boophilus microplus using biochemical and molecular tests to investigate the mechanisms conferring acaricide resistance. The electrophoretic profiles of esterase activity in protein extracts from coumaphos and permethrin-resistant strains compared to the susceptible strain revealed distinct differences, which inhibitor studies attributed to carboxylesterases. Esterase hydrolysis assays showed significant enhancement of both total and permethrin hydrolysis in one pyrethroid-resistant strain, with no enhancement in two other strains with very high resistance to pyrethroids. Sequence analysis of sodium channel mRNA fragments in all pyrethroid-resistant strains determined that they did not possess the classic kdr and super-kdr mutations known to confer pyrethroid resistance in several insect species. Using reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed from conserved regions of insect esterase amino acid sequences, a B. microplus larval cDNA fragment was isolated whose deduced amino acid sequence was significantly similar to esterases from a wide range of species. In Northern blot RNA analysis the cDNA hybridized to a 2.1 kb mRNA that was abundant in all resistant strains except one, in which a very low abundance could provide a marker for the mechanism conferring resistance in this strain. Published by Elsevier Science Ltd.
引用
收藏
页码:685 / 695
页数:11
相关论文
共 35 条
[1]   Acetylcholinesterase cDNA of the cattle tick, Boophilus microplus:: characterisation and role in organophosphate resistance [J].
Baxter, GD ;
Barker, SC .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1998, 28 (08) :581-589
[2]   METABOLISM OF COUMAPHOS IN SUSCEPTIBLE AND RESISTANT STRAINS OF BOOPHILUS-MICROPLUS (ACARI, IXODIDAE) [J].
BULL, DL ;
AHRENS, EH .
JOURNAL OF MEDICAL ENTOMOLOGY, 1988, 25 (02) :94-98
[3]   Expressed sequenced tags and new genes from the cattle tick, Boophilus microplus [J].
Crampton, AL ;
Miller, C ;
Baxter, GD ;
Barker, SC .
EXPERIMENTAL & APPLIED ACAROLOGY, 1998, 22 (03) :177-186
[4]  
CYGLER M, 1993, PROTEIN SCI, V2, P366
[5]   In vitro and in vivo evaluations of a strain of Boophilus microplus (Acari: Ixodidae) selected for resistance to permethrin [J].
Davey, RB ;
George, JE .
JOURNAL OF MEDICAL ENTOMOLOGY, 1998, 35 (06) :1013-1019
[6]   OVIPOSITIONAL BIOLOGY OF THE CATTLE TICK, BOOPHILUS-ANNULATUS (ACARI, IXODIDAE), IN THE LABORATORY [J].
DAVEY, RB ;
GARZA, J ;
THOMPSON, GD ;
DRUMMOND, RO .
JOURNAL OF MEDICAL ENTOMOLOGY, 1980, 17 (03) :287-289
[7]   HOST-RESISTANCE IN CATTLE TICK CONTROL [J].
DECASTRO, JJ ;
NEWSON, RM .
PARASITOLOGY TODAY, 1993, 9 (01) :13-17
[8]   DETECTION OF INSECTICIDE RESISTANCE BY IMMUNOLOGICAL ESTIMATION OF CARBOXYLESTERASE ACTIVITY IN MYZUS-PERSICAE (SULZER) AND CROSS REACTION OF THE ANTISERUM WITH PHORODON-HUMULI (SCHRANK) (HEMIPTERA, APHIDIDAE) [J].
DEVONSHIRE, AL ;
MOORES, GD ;
FFRENCHCONSTANT, RH .
BULLETIN OF ENTOMOLOGICAL RESEARCH, 1986, 76 (01) :97-107
[9]  
FIELD LM, 1996, MOL GENETICS EVOLUTI, P72
[10]  
Fournier D, 1996, ACS SYM SER, V645, P17