Do conjugated eicosapentaenoic acid and conjugated docosahexaenoic acid induce apoptosis via lipid peroxidation in cultured human tumor cells?

被引:63
作者
Igarashi, M [1 ]
Miyazawa, T [1 ]
机构
[1] Tohoku Univ, Grad Sch Life Sci & Agr, Chem Biodynam Lab, Sendai, Miyagi 9818555, Japan
关键词
conjugated fatty acid; eicosapentaenoic acid; docosahexaenoic acid; human tumor cells; fibroblast; lipid hydroperoxide; apoptosis;
D O I
10.1006/bbrc.2000.2484
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Conjugated eicosapentaenoic acid (CEPA) and conjugated docosahexaenoic acid (CDHA) with triene structure, isomerized by alkaline treatment, showed intensive cytotoxicity with LD50 at 12 and 16 mu M, respectively, in DLD-1 cells (colorectal adenocarcinoma), while they had no effect on normal human fibroblast cell lines such as MRC-5, TIG-103, and KMS-6 cells, Cytotoxic action of CEPA and CDHA was also demonstrated in other tumor cell lines including HepG2, A549, MCF-7, and MKN-7 cells. cu-Tocopherol suppressed cytotoxicity of CEPA and CDHA in tumor cells, and the cytotoxicity involved membrane phospholipid peroxidation. CEPA and CDHA induced DNA condensation and fragmentation in DLD-1 cells, indicating the involvement of apoptosis in this cytotoxic mechanism. Furthermore, previous reports have shown that lipid peroxidation product induces cell death, including apoptotic cell death in different cell lines. CEPA and CDHA have been demonstrated in cultured cells to cause cell death via lipid peroxidation and apoptosis in the absence of cu-tocopherol. (C) 2000 Academic Press.
引用
收藏
页码:649 / 656
页数:8
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