A critical temporal window for selectin-dependent CD4+ lymphocyte homing and initiation of late-phase inflammation in contact sensitivity

被引:50
作者
Hwang, JM
Yamanouchi, J
Santamaria, P
Kubes, P
机构
[1] Univ Calgary, Dept Physiol & Biophys, Immunol Res Grp, Calgary, AB T2N 4N1, Canada
[2] Univ Calgary, Fac Med, Dept Infect Dis & Microbiol, Calgary, AB T2N 4N1, Canada
关键词
delayed hypersensitivity; contact dematitis; neutrophils; vascular cell adhesion molecule-1; CD4(+) T lymphocytes;
D O I
10.1084/jem.20032016
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Contact sensitivity (CS) is all inflammatory disorder characterized by early and late phases of leukocyte recruitment. We used a noninvasive intravital microscopy technique allowing for the direct visualization of leukocyte rolling and adhesion on blood vessel endothelium. By blocking specific adhesion molecules, we elucidated the molecular mechanisms mediating early leukocyte recruitment to be E- and P-selectin and demonstrated that leukocyte recruitment in the late phase had a different adhesive profile (mainly alpha(4)-integrin). Complete blockade of E- and P-selectin within the first 2 h of leukocyte-endothelial cell interactions (but not later) eliminated selectin-independent leukocyte recruitment at 24 h. Despite the predominance of neutrophils in the early phase, specific elimination of CD4(+) lymphocytes in the early phase eliminated the late response. CD4(+) lymphocytes horned to skin via E- and P-selectin within the early phase and induced the late phase response. Addition of these same CD4(+) lymphocytes 2 h after antigen challenge was too late for these cells to home to the skin and induce late phase responses. Our data clearly demonstrate that the antigen-challenged microenvironment is only accessible to CD+ lymphocytes for the first 2 h, and that this process is essential for the subsequent recruitment of other leukocyte populations in late phase responses.
引用
收藏
页码:1223 / 1234
页数:12
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