Metabolic relations of inositol 3,4,5,6-tetrakisphosphate revealed by cell permeabilization. Identification of inositol 3,4,5,6-tetrakisphosphate 1-kinase and inositol 3,4,5,6-tetrakisphosphate phosphatase activities in mesophyll cells

被引:21
作者
Brearley, CA [1 ]
Hanke, DE [1 ]
机构
[1] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England
关键词
D O I
10.1104/pp.122.4.1209
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Using a permeabilization strategy to introduce Ins(3,4,5,6) P-4 into mesophyll protoplasts of Commelina communis, we have identified Ins(3,4,5,6) P-4 l-kinase activity in mesophyll cells. Multiple InsP(3) isomers were identified in Spirodela polyrhiza and Arabidopsis. Only two of these, Ins(1,2,3) P-3 and Ins(3,4,6) P-3, have previously been identified in plants and only in monocots. The isomers detected in S. polyrhiza included D- and/or L-Ins(3,4,5) P-3, D- and/or L-Ins(3,5,6) P-3, and D- and/or L-Ins(2,4,5) P-3. Ins(1,4,5) P-3, if present, was only a tiny fraction of total InsP(3) species. We have also identified inositol polyphosphate phosphatase activities, Ins(3,4,5,6) P-4 6-phosphatase and Ins(3,4,5, 6) P-4 4-phosphatase, whose action on endogenous inositol polyphosphates explains the presence of D- and/or L-Ins(3,4,5) P-3 and D- and/or L-Ins(3,5,6) P-4 in mesophyll cells. Inositol trisphosphates identified in Arabidopsis include Ins(1,2,3) P-3 and D- and/or L-Ins(3,4,6) P-3, suggesting that dicots may share pathways of InsP(6) biosynthesis and breakdown in common with monocots.
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页码:1209 / 1216
页数:8
相关论文
共 27 条
[21]   PRODUCT PRECURSOR RELATIONSHIPS AMONGST INOSITOL POLYPHOSPHATES - INCORPORATION OF [P-32] PI INTO MYOINOSITOL 1,3,4,6-TETRAKISPHOSPHATE, MYOINOSITOL 1,3,4,5-TETRAKISPHOSPHATE, MYOINOSITOL 3,4,5,6-TETRAKISPHOSPHATE AND MYOINOSITOL 1,3,4,5,6-PENTAKISPHOSPHATE IN INTACT AVIAN ERYTHROCYTES [J].
STEPHENS, LR ;
DOWNES, CP .
BIOCHEMICAL JOURNAL, 1990, 265 (02) :435-452
[22]  
TOMLINSON R, 1961, J BIOL CHEM, V236, P1902
[23]   MYOINOSITOL POLYPHOSPHATE INTERMEDIATES IN DEPHOSPHORYLATION OF PHYTIC ACID BY PHYTASE [J].
TOMLINSON, RV ;
BALLOU, CE .
BIOCHEMISTRY, 1962, 1 (01) :166-&
[24]   Nucleus-associated phosphorylation of Ins(1,4,5)P-3 to InsP(6) in Dictyostelium [J].
VanderKaay, J ;
Wesseling, J ;
VanHaastert, JM .
BIOCHEMICAL JOURNAL, 1995, 312 :911-917
[25]   Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning, and expression of the recombinant enzyme [J].
Wilson, MP ;
Majerus, PW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (20) :11904-11910
[26]   Characterization of a cDNA encoding Arabidopsis thaliana inositol 1,3,4-trisphosphate 5/6-kinase [J].
Wilson, MP ;
Majerus, PW .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 232 (03) :678-681
[27]   THE INOSITOL PHOSPHATES IN WRK1 RAT MAMMARY-TUMOR CELLS [J].
WONG, NS ;
BARKER, CJ ;
MORRIS, AJ ;
CRAXTON, A ;
KIRK, CJ ;
MICHELL, RH .
BIOCHEMICAL JOURNAL, 1992, 286 :459-468