Hydrogen peroxide induces apoptosis of osteocytes: Involvement of calcium ion and caspase activity

被引：27

作者：

Kikuyama, A ^{[1
]}

Fukuda, K ^{[1
]}

Mori, S ^{[1
]}

Okada, M ^{[1
]}

Yamaguchi, H ^{[1
]}

Hamanishi, C ^{[1
]}

机构：

[1] Kinki Univ, Sch Med, Dept Orthopaed Surg, Osaka 5898511, Japan

来源：

CALCIFIED TISSUE INTERNATIONAL | 2002年 / 71卷 / 03期

关键词：

hydrogen peroxide; caspase-3; cystolic Ca2+; apoptosis; osteocytes;

D O I：

10.1007/s00223-001-1110-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide. annexin V binding., which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the Ca2+ activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.

引用

页码：243 / 248

页数：6

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