IDENTIFICATION AND INHIBITION OF THE ICE/CED-3 PROTEASE NECESSARY FOR MAMMALIAN APOPTOSIS

被引：3785

作者：

NICHOLSON, DW

ALI, A

THORNBERRY, NA

VAILLANCOURT, JP

DING, CK

GALLANT, M

GAREAU, Y

GRIFFIN, PR

LABELLE, M

LAZEBNIK, YA

MUNDAY, NA

RAJU, SM

SMULSON, ME

YAMIN, TT

YU, VL

MILLER, DK

机构：

[1] MERCK FROSST CANADA INC,MERCK FROSST CTR THERAPEUT RES,DEPT MED CHEM,POINTE CLAIRE,PQ H9R 4P8,CANADA

[2] MERCK & CO INC,RES LABS,DEPT BIOCHEM,RAHWAY,NJ 07065

[3] MERCK & CO INC,RES LABS,DEPT INFLAMMAT RES,RAHWAY,NJ 07065

[4] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724

[5] GEORGETOWN UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOL,WASHINGTON,DC

来源：

NATURE | 1995年 / 376卷 / 6535期

关键词：

D O I：

10.1038/376037a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The protease responsible for the cleavage of poly(ADP-ribose) polymerase and necessary for apoptosis has been purified and characterized. This enzyme, named apopain, is composed of two subunits of relative molecular mass (M(r)) 17K and 12K that are derived from a common proenzyme identified as CPP32. This proenzyme is related to interleukin-1 beta-converting enzyme (ICE) and CED-3, the product of a gene required for programmed cell death in Caenorhabditis elegans. A potent peptide aldehyde inhibitor has been developed and shown to prevent apoptotic events In vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death.

引用

页码：37 / 43

页数：7

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