Efficient transduction of mature CD83+ dendritic cells using recombinant adenovirus suppressed T cell stimulatory capacity

被引：49

作者：

Jonuleit, H ^{[1
]}

Tüting, T ^{[1
]}

Steitz, J ^{[1
]}

Brück, J ^{[1
]}

Giesecke, A ^{[1
]}

Steinbrink, K ^{[1
]}

Knop, J ^{[1
]}

Enk, AH ^{[1
]}

机构：

[1] Univ Mainz, Dept Dermatol, D-55101 Mainz, Germany

来源：

GENE THERAPY | 2000年 / 7卷 / 03期

关键词：

dendritic cells; genetic immunization; recombinant adenovirus;

D O I：

10.1038/sj.gt.3301077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a culture method for the foreign serum-free generation of highly immunostimulatory, CD83(+) human dendritic cells (DC). In this study, we evaluated the feasibility and consequences of endogenously expressing antigens in mature DC using adenoviral vectors. Transduction of DC with Ad-EGFP demonstrated endogenous fluorescence in 50-85% of CD83(+) DC. Ad-transduced DC stimulated the proliferation of allogeneic CD8(+) and CD4(+) T cells at low DC: T cell ratios. However, at high DC: T cell ratios the stimulatory capacity of Ad-transduced DC was suppressed. This immunosuppressive effect was confirmed by demonstrating that the stimulatory function of untreated DC could be suppressed in a dose-dependent manner by addition of Ad-transduced DC. Furthermore, transwell experiments suggested that direct cell contact was required. Taken together our results demonstrate the feasibility of efficiently expressing antigens in CD83(+) DC using adenoviruses. However, immunosuppressive effects must be considered and carefully studied before Ad-transduced DC are employed for clinical trials.

引用

页码：249 / 254

页数：6

共 20 条

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