NMR-based metabolomics

被引:201
作者
Reo, NV [1 ]
机构
[1] Wright State Univ, Sch Med, Dept Biochem & Mol Biol, WSU Magnet Resonance Lab,Cox Inst, Dayton, OH 45429 USA
关键词
NMR; metabonomics; metabolomics;
D O I
10.1081/DCT-120014789
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Similar to genomics and proteomics which yield vast amounts of data about the expression of genes and proteins, metabolomics refers to the whole metabolic profile of the cell. The focus of this report concerns the use of nuclear magnetic resonance (NMR) spectroscopy for metabolic analyses and, in particular, its use in toxicology for examining the metabolic profile of biofluids. Examples from the literature will demonstrate how H-1 NMR and pattern recognition methods are used to obtain the urinary metabolic profile, and how this profile is affected by exposure to various toxicants. These particular studies which focus on the metabolic profiles of biofluids, specifically urine, are referred to as metabonomics. NMR-based metabonomics provides a means to categorize organ-specific toxicity, monitor the onset and progression of toxicological effects, and identify biomarkers of toxicity. A future challenge, however, is to describe the cellular metabolome for purposes of understanding cellular functions (i.e., metabolomics). Thus the capabilities and advantages of multinuclear NMR to provide metabolic information in cells and tissues will also be discussed. Such information is essential if metabolomics is to provide a complementary dataset which together with genomics and proteomics can be used to construct computer network models to describe cellular functions.
引用
收藏
页码:375 / 382
页数:8
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