Phosphate sensing system using pyruvate oxidase and chemiluminescence detection

被引：35

作者：

Ikebukuro, K

Wakamura, H

Karube, I

Kubo, I

Inagawa, M

Sugawara, T

Arikawa, Y

Suzuki, M

Takeuchi, T

机构：

[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,MEGURO KU,TOKYO 153,JAPAN

[2] SOKA UNIV,FAC ENGN,DEPT BIOENGN,HACHIOJI,TOKYO 192,JAPAN

[3] JAPAN WOMENS UNIV,FAC SCI,DEPT CHEM & BIOL SCI,BUNKYO KU,TOKYO 112,JAPAN

[4] KYUSHU INST TECHNOL,FAC COMP SCI & SYST ENGN,DEPT BIOCHEM ENGN SCI,IIZUKA,FUKUOKA 820,JAPAN

[5] HIROSHIMA CITY UNIV,FAC INFORMAT SCI,ASANAMI KU,HIROSHIMA 73113,JAPAN

来源：

BIOSENSORS & BIOELECTRONICS | 1996年 / 11卷 / 10期

关键词：

phosphate; luminol; chemiluminescence; pyruvate oxidase; flow injection analysis;

D O I：

10.1016/0956-5663(96)87655-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A flow injection phosphate analysis system based on an enzymic reaction and a subsequent luminol chemiluminescence reaction has been developed. The system consists of an immobilized pyruvate oxidase column, mixing chamber for the chemiluminescent reaction and a photomultiplier. The H2O2 generated by the reaction of phosphate and pyruvate oxidase then reacts with luminol and horseradish peroxidase and the consequent chemiluminescence is detected using a photomultiplier. This system is capable of the rapid determination of phosphate, the time required for one measurement cycle being approximately 3 min. A linear response was observed from 4.8 to 160 mu M phosphate. (C) 1996 Elsevier Science Limited.

引用

页码：959 / 965

页数：7

共 15 条

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