Expression of cytokeratins in human enamel organ

被引:69
作者
Domingues, MG
Jaeger, MMM
Araújo, VC
Araújo, NS
机构
[1] Univ Sao Paulo, Fac Odontol, Disciplina Patol Bucal, Dept Oral Pathol, BR-05508900 Sao Paulo, Brazil
[2] Ibirapuera Univ, Sch Dent, Dept Oral Pathol, Sao Paulo, Brazil
关键词
odontogenesis; odontogenic epithelia; enamel organ; cytokeratin; immunohistochemistry;
D O I
10.1034/j.1600-0722.2000.00717.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Cytokeratin (CK) is a filament which plays a central role in epithelial tissue and, like the polypeptides of intermediate filaments in general, shows a high degree of tissue specificity. The CK expression patterns of odontogenic epithelia are still poorly described. We studied the distribution of individual CK polypeptides in the human enamel organ at bell stage and in remnants of the dental lamina. Our immunohistochemical study showed that epithelial cells stained for CKs 7, 13, 14 and 19 with slight changes in their pattern during the differentiation phase of odontogenesis. There was negative staining for all other CK polypeptides tested (CKs 8, 10, 16, 17 and 18). Most of the CKs in the enamel organ epithelia did not show differences related to the stage-specific state of differentiation, except for CKs 14 and 19 at the inner enamel epithelium. A strong label for CK 14 was present at the inner dental epithelium at early bell stage, and this was substituted by CK 19 at the late bell stage when the ameloblasts were fully differentiated.
引用
收藏
页码:43 / 47
页数:5
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