Cationic oligonucleotide-peptide conjugates with aggregating properties enter efficiently into cells while maintaining hybridization properties and enzymatic recognition

被引：44

作者：

Fraley, Andrew W. ^{[1
]}

Pons, Benedicte ^{[1
]}

Dalkara, Deniz ^{[1
]}

Nullans, Gerard ^{[1
]}

Behr, Jean-Paul ^{[1
]}

Zuber, Guy ^{[1
]}

机构：

[1] Univ Louis Pasteur Strasbourg 1, Lab Chim Genet, CNRS, Fac Pharm, F-67401 Illkirch Graffenstaden, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 33期

关键词：

D O I：

10.1021/ja060873e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oligonucleotide delivery is a crucial issue for therapeutical purposes and is often addressed by conjugation to short cationic peptides although with controversial results. To further examine this mechanism, a 15-mer anionic oligonucleotide was conjugated to a cationic peptide in order to obtain a diblock compound with an overall positive charge with aggregation properties. These microaggregates were efficiently internalized in cells via the expeditious pathway used by commercial gene delivery systems. Moreover, stability of the duplex formed with the complementary sequence increased without inhibiting oligonucleotide enzyme recognition as shown by the properties of the conjugate to prime chain elongation by Taq DNA polymerase in a linear amplification/sequencing process.

引用

页码：10763 / 10771

页数：9

共 38 条

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