Secreted Klotho protein in sera and CSF: implication for post-translational cleavage in release of Klotho protein from cell membrane

被引：436

作者：

Imura, A

Iwano, A

Tohyama, O

Tsuji, Y

Nozaki, K

Hashimoto, N

Fujimori, T

Nabeshima, Y ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Pathol & Tumor Biol, Sakyo Ku, Kyoto 6068501, Japan

[2] Core Res Ecolut Sci & Technol, Kawaguchi 3320012, Japan

[3] Kyoto Univ, Horizontal Med Res Org, Sakyo Ku, Kyoto 6068501, Japan

[4] Kyoto Univ, Grad Sch Med, Dept Neurosurg, Sakyo Ku, Kyoto 6068501, Japan

来源：

FEBS LETTERS | 2004年 / 565卷 / 1-3期

关键词：

klotho protein; humoral factor; N-glycosylation; post-translational cleavage;

D O I：

10.1016/j.febslet.2004.03.090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Klotho mutant mice exhibit a set of phenotypes resembling human ageing. Although the function of Klotho remains unclear, mediation of its pleiotropic functions by putative Immoral factor(s) has been presumed. Newly established antibodies against Klotho allowed the detection of secreted Klotho, a candidate for the putative Immoral factor, in sera and cerebrospinal fluid. Surprisingly the secreted Klotho was 130 kDa, in contrast to the 70 kDa predicted form from klotho gene transcripts. The secreted as well as the membrane-bound Klotho proteins were suggested to form oligomerized complex. These results delineate post-translation processing of Klotho and possible regulatory. mechanisms for secretion of Klotho in vivo. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：143 / 147

页数：5