In-depth characterization of slurry packed capillary columns with 1.0-μm nonporous particles using reversed-phase isocratic ultrahigh-pressure liquid chromatography

被引：161

作者：

Patel, KD ^{[1
]}

Jerkovich, AD ^{[1
]}

Link, JC ^{[1
]}

Jorgenson, JW ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 19期

关键词：

D O I：

10.1021/ac049756x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fused-silica capillary columns packed with 1.0-mum nonporous C-18 bonded particles are evaluated with isocratic ultrahigh-pressure liquid chromatography (UHPLC). Improved UHPLC techniques have demonstrated column efficiencies as high as 730 000 plates/m and run pressures over 6800 bar (100 000 psi) for packed 10-mum-inner diameter (i.d.) columns. Columns as large as 150 mum have been tested with UHPLC and show no flow-induced heating effects on separation efficiencies. van Deemter plot analysis for column i.d.s ranging from 10 to 150 pm shows an increase in column efficiency with a decrease in column i.d.. Reduced parameter analysis further illustrates a decrease in reduced parameter A term and C term values with decreasing W. However, reduced parameter C term values for columns evaluated with UHPLC are an order of magnitude larger than C term values for larger particles at conventional pressures. Retention factors for moderately retained compounds are observed to increase with column i.d., suggesting an increase in packing density. Highly ordered packing arrangement at the column wall is seen for packed beds extruded from large-diameter columns.

引用

页码：5777 / 5786

页数：10

共 42 条

[21] B-TERM AND C-TERM IN THE VANDEEMTER EQUATION FOR LIQUID-CHROMATOGRAPHY [J].

KNOX, JH ;

SCOTT, HP .

JOURNAL OF CHROMATOGRAPHY, 1983, 282 (DEC) :297-313

[22] Accuracy of empirical correlations for estimating diffusion coefficients in aqueous organic mixtures [J].

Li, JW ;

Carr, PW .

ANALYTICAL CHEMISTRY, 1997, 69 (13) :2530-2536

[23]

Lide D. R, 1992, CRC Handbook of Chemistry and Physics

[24]

Lippert JA, 1999, J MICROCOLUMN SEP, V11, P631, DOI 10.1002/(SICI)1520-667X(199911)11:9<631::AID-MCS1>3.3.CO

[25]

2-9

[26]

MacNair JE, 1997, RAPID COMMUN MASS SP, V11, P1279, DOI 10.1002/(SICI)1097-0231(199708)11:12<1279::AID-RCM18>3.0.CO

[27]

2-S

[28] Ultrahigh pressure reversed-phase capillary liquid chromatography:: Isocratic and gradient elution using columns packed with 1.0-μm particles [J].

MacNair, JE ;

Patel, KD ;

Jorgenson, JW .

ANALYTICAL CHEMISTRY, 1999, 71 (03) :700-708

[29] Ultrahigh pressure reversed-phase liquid chromatography in packed capillary columns [J].

MacNair, JE ;

Lewis, KC ;

Jorgenson, JW .

ANALYTICAL CHEMISTRY, 1997, 69 (06) :983-989

[30]

MACNAIR JE, 1998, THESIS U N CAROLINA

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