Small interfering RNA (siRNA) targetted to Smad3 inhibits transforming growth factor-β signaling

被引：31

作者：

Yeom, SY

Jeoung, D

Ha, KS

Kim, PH ^{[1
]}

机构：

[1] Kangweon Natl Univ, Coll Nat Sci, Dept Microbiol, Chunchon 200701, South Korea

[2] Kangweon Natl Univ, Vasc Syst Res Ctr, Chunchon 200701, South Korea

[3] Kangweon Natl Univ, Sch Med, Dept Mol & Cellular Biochem, Chunchon 200701, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 09期

关键词：

anti-proliferative activity; luciferase assay; Smad3; protein; small interfering RNA; transforming growth factor-beta;

D O I：

10.1023/B:BILE.0000024091.90305.be

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

RNA interference has become a powerful tool for silencing of gene expression in mammals and plants. To determine the effect of Smad3 on transforming growth factor-beta signaling, we constructed a small interfering RNA (siRNA) targeted to Smad3. This siRNA inhibited expression of the endogenous Smad3 leading to the prevention of nuclear localization of Smad3. Further, Smad3 siRNA prevented not only anti-proliferative activity of TGF-beta1 but also TGF-beta1-inducible promoter activity.

引用

页码：699 / 703

页数：5

共 13 条

[1]

Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines [J].