A microarray-based method for detecting methylated loci

被引：40

作者：

Hatada, I

Kato, A

Morita, S

Obata, Y

Nagaoka, K

Sakurada, A

Sato, M

Horii, A

Tsujimoto, A

Matsubara, K

机构：

[1] Gunma Univ, Ctr Gene Res, Maebashi, Gumma 3718511, Japan

[2] DNA Chip Res Inc, Yokohama, Kanagawa, Japan

[3] Tohoku Univ, Dept Thorac Surg, Inst Dev Aging & Canc, Sendai, Miyagi 980, Japan

[4] Tohoku Univ, Dept Mol Pathol, Sch Med, Sendai, Miyagi 980, Japan

[5] Nara Inst Sci & Technol, Taisho Lab Funct Genom, Ikoma, Japan

来源：

JOURNAL OF HUMAN GENETICS | 2002年 / 47卷 / 08期

关键词：

DNA methylation; DNA chip; microarray; CpG island; hypermethylation;

D O I：

10.1007/s100380200063

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

CpG island DNA methylation plays an important role in regulating gene expression in development and carcinogenesis. We developed a new microarray-based method called methylation amplification DNA chip (MAD) for detecting differences in methylation. In this method, only methylated CpG islands from the two samples that we wanted to compare were amplified and used for hybridization. The resource material for the microarray was derived from the methylated DNA library of the sample in which we wanted to detect hypermethylation. Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray.

引用

页码：448 / 451

页数：4

共 13 条

[1] HIGH-LEVELS OF DENOVO METHYLATION AND ALTERED CHROMATIN STRUCTURE AT CPG ISLANDS IN CELL-LINES [J].