Fluorescent proteins: powerful tools in phagocyte biology

被引：9

作者：

Bajno, L

Grinstein, S

机构：

[1] Hosp Sick Children, Cell Biol Programme, Inst Res, Toronto, ON M5G 1X8, Canada

[2] Univ Toronto, Dept Biochem, Toronto, ON, Canada

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 232卷 / 1-2期

基金：

加拿大自然科学与工程研究理事会; 英国医学研究理事会;

关键词：

fluorescent protein; GFP; imaging; FRET; FRAP; chemotaxis; phagocytosis;

D O I：

10.1016/S0022-1759(99)00155-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phagocyte functions such as chemotaxis and phagocytosis involve the rapid and transient development of cellular polarity. Study of this highly complex spatial and temporal cellular remodelling has been limited by the static nature of immunofluorescence and immunogold microscopy and because biochemical techniques are not vectorial. The recent introduction Of fluorescent proteins (FPs) provides new approaches and opportunities to study phagocyte functions non-invasively, with excellent temporal and spatial resolution, This review summarizes the main properties and possible uses of green fluorescent protein (GFP) and its variants in phagocyte biology. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：67 / 75

页数：9

共 40 条

[1] Mechanisms of epithelial cell-cell adhesion and cell compaction revealed by high-resolution tracking of E-cadherin-green fluorescent protein [J].