Aurora B TACC1 protein complex in cytokinesis

被引：42

作者：

Delaval, B

Ferrand, A

Conte, N

Larroque, C

Hernandez-Verdun, D

Prigent, C

Birnbaum, D

机构：

[1] Inst J Paoli I Calmettes, INSERM, U119, Dept Mol Oncol, F-13009 Marseille, France

[2] CRLC Val Aurelle Paul Lamarque, INSERM, E229, Montpellier, France

[3] Univ Rennes 1, Fac Med, CNRS,UMR Genet & Dev 6061, Grp Cycle Cellulaire, Rennes, France

[4] Inst Jacques Monod, CNRS, F-75251 Paris, France

来源：

ONCOGENE | 2004年 / 23卷 / 26期

关键词：

Aurora kinase; cell division; cytokinesis; microtubule; midbody; TACC protein; Taxin; RNAi;

D O I：

10.1038/sj.onc.1207593

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taxins are a family of centrosomal proteins important for the regulation of mitosis and microtubule dynamics. Cytokinesis, the last step of M phase, is essential for chromosomal integrity and cell division. It is highly regulated and involves a reorganization of microtubules and actin filaments. We show here that TACC1 localizes diffusely to the midzone spindle in anaphase and strongly to the midbody during cytokinesis, indicating a possible involvement of this protein in the exit of M phase. TACC1 also relocalizes to the nucleolus in interphase. We demonstrate that TACC1 and the mitotic kinase Aurora B belong to the same complex during cytokinesis. We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody-and consequently affects TACC1 localization at this site-and leads to abnormal cell division and multinucleated cells.

引用

收藏

页码：4516 / 4522

页数：7

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