Synthesis of a chemiluminescent acridinium hydroxylamine (AHA) for the direct detection of abasic sites in DNA

被引：14

作者：

Adamczyk, M ^{[1
]}

Mattingly, PG ^{[1
]}

Moore, JA ^{[1
]}

Pan, Y ^{[1
]}

机构：

[1] Abbott Labs, Dept Chem, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

ORGANIC LETTERS | 1999年 / 1卷 / 05期

关键词：

D O I：

10.1021/ol990141+

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] The synthesis of a chemiluminescent acridinium hydroxylamine (AHA) for the direct detection of abasic sites in damaged nucleic acids is described, The reagent reacts readily with abasic sites of damaged calf thymus DNA generated in a time dependent manner under acid/heat depurination conditions, Preliminary results indicate the sensitivity of the direct chemiluminescent detection format is similar to 0.1 abasic sites detected per 10(6) nucleotides using as little as 200 ng of DNA.

引用

页码：779 / 781

页数：3

共 17 条

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