Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates

被引：94

作者：

Belitsky, JM

Leslie, SJ

Arora, PS

Beerman, TA

Dervan, PB ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] CALTECH, Beckman Inst, Pasadena, CA 91125 USA

[3] Roswell Pk Canc Inst, Dept Pharmacol & Therapeut, Buffalo, NY 14263 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2002年 / 10卷 / 10期

关键词：

D O I：

10.1016/S0968-0896(02)00204-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide-dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide-dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：3313 / 3318

页数：6

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