Triplex staples: DNA double-strand cross-linking at internal and terminal sites using psoralen-containing triplex-forming oligonucleotides

被引：29

作者：

Li, Hong

Broughton-Head, Victoria J.

Peng, Guomei

Powers, Vicki E. C.

Ovens, Matthew J.

Fox, Keith R.

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Southampton, Sch Biol Sci, Southampton SO16 7PX, Hants, England

来源：

BIOCONJUGATE CHEMISTRY | 2006年 / 17卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1021/bc0601875

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed to attach 4'-( hydroxymethyl)- 4,5', 8- trimethylpsoralen to the 5 position of thymine bases during solid- phase oligonucleotide synthesis. UV irradiation of triplex- forming oligonucleotides ( TFOs) containing internally attached psoralens produces photoadducts at TpA steps within target duplexes, thus relaxing the constraints on selection of psoralen target sequences. Photoreaction of TFOs containing two psoralens, located at the 5'- and 3'- ends, has been used to create double-strand cross- links ( triplex staples) at both termini of the TFO. Such complexes have no free single-stranded ends. TFOs containing 4'-( hydroxymethyl)- 4,5', 8- trimethylpsoralen, 3- methyl- 2- aminopyridine, and 5-( 3- aminoprop- 2- ynyl) deoxyuridine formed photoadducts with target duplexes under near- physiological conditions.

引用

页码：1561 / 1567

页数：7

共 25 条

[11] 5-substituted 2-aminopyridine C-nucleosides as protonated cytidine equivalents: Increasing efficiency and selectivity in DNA triple-helix formation [J].