Layer-by-Layer Assembled Gold Nanoparticles for siRNA Delivery

被引：354

作者：

Elbakry, Asmaa ^{[1
]}

Zaky, Alaa ^{[1
]}

Liebkl, Renate ^{[1
]}

Rachel, Reinhard ^{[2
]}

Goepferich, Achim ^{[1
]}

Breunig, Miriam ^{[1
]}

机构：

[1] Univ Regensburg, Dept Pharmaceut Technol, D-93040 Regensburg, Germany

[2] Univ Regensburg, Inst Anat, Ctr Electron Microscopy, D-93040 Regensburg, Germany

来源：

NANO LETTERS | 2009年 / 9卷 / 05期

关键词：

FUNCTIONAL CORE/SHELL NANOPARTICLES; MAMMALIAN-CELLS; GENE-REGULATION; DNA COMPLEXES; QUANTUM DOTS; OLIGONUCLEOTIDES; THERAPEUTICS; POLYPLEXES; ANTISENSE; SIZE;

D O I：

10.1021/nl9003865

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Although uptake into cells is highly complex and regulated, heterogeneous particle collectives are usually employed to deliver small interfering RNA (siRNA) to cells. Within these collectives, it is difficult to accurately identify the active species, and a decrease in efficacy is inherent to such preparations. Here, we demonstrate the manufacture of uniform nanoparticles with the deposition of siRNA on gold in a layer-by-layer approach, and we further report on the cellular delivery and siRNA activity as functions of surface properties.

引用

页码：2059 / 2064

页数：6

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