Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells

被引：85

作者：

Cambi, Alessandra ^{[1
]}

Lidke, Diane S.

Arndt-Jovin, Donna J.

Figdor, Carl G.

Jovin, Thomas M.

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Biol, D-37077 Gottingen, Germany

[2] Radboud Univ Nijmegen Med Ctr, Dept Tumor Immunol, Ctr Mol Life Sci, Nijmegen, Netherlands

来源：

NANO LETTERS | 2007年 / 7卷 / 04期

关键词：

LECTIN DC-SIGN; T-CELLS; LIVE CELLS; RECEPTOR; VIRUS; INFECTION; TRANS; VACCINATION; ANISOTROPY; RESPONSES;

D O I：

10.1021/nl0700503

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.

引用

页码：970 / 977

页数：8

共 37 条

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