Noninvasive label-free imaging of microhemodynamics by optical-resolution photoacoustic microscopy

被引:91
作者
Hu, Song [1 ]
Maslov, Konstantin [1 ]
Wang, Lihong V. [1 ]
机构
[1] Washington Univ, Opt Imaging Lab, Dept Biomed Engn, St Louis, MO 63130 USA
来源
OPTICS EXPRESS | 2009年 / 17卷 / 09期
基金
美国国家卫生研究院;
关键词
BLOOD-FLOW; MICROCIRCULATION; VASOMOTION; CAPILLARIES; HYPEROXIA; HYPOXIA; DISEASE; CANCER;
D O I
10.1364/OE.17.007688
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
In vivo microcirculatory imaging facilitates the fundamental understanding of many major diseases. However, existing techniques generally require invasive procedures or exogenous contrast agents, which perturb the intrinsic physiology of the microcirculation. Here, we report on optical-resolution photoacoustic microscopy (OR-PAM) for noninvasive label-free microcirculatory imaging at cellular levels. For the first time, OR-PAM demonstrates quantification of hemoglobin concentration and oxygenation in single microvessels down to capillaries. Using this technique, we imaged several important yet elusive microhemodynamic activities-including vasomotion and vasodilation-in small animals in vivo. OR-PAM enables functional volumetric imaging of the intact microcirculation, thereby providing greatly improved accuracy and versatility for broad biological and clinical applications. (C) 2009 Optical Society of America
引用
收藏
页码:7688 / 7693
页数:6
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