MEK inhibition and phosphorylation of serine 4 on B23 are two coincident events in mitosis

被引：11

作者：

Hayne, C ^{[1
]}

Xiang, XQ ^{[1
]}

Luo, ZJ ^{[1
]}

机构：

[1] Boston Univ, Sch Med, Dept Med, Sect Endocrinol,Diabet Res Unit, Boston, MA 02118 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 321卷 / 03期

关键词：

Raf; MEK; ERK; cell cycle; mitosis; B23/numatrin/nucleophosmin; PLK1; CDK;

D O I：

10.1016/j.bbrc.2004.07.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have shown that activation of the Raf/MEK/ERK pathway is necessary for G2/M transition. However, as for the activation state of MEK in mitosis the conclusion is not consistent. Here we show that MEK is inhibited in mitosis. In addition, we identify a multifunctional protein named B23 that strongly cross-reacts with a phospho-MEK antibody in mitotic cells. Sequence homology between the N-terminus surrounding Ser 4 of B23 and the Raf phosphorylation site on MEK suggests a mechanism for cross-reaction of the antibody. Thus, mutation of Ser 4 to alanine abolishes cross-reactivity between B23 and the phospho-MEK antibody. Our findings may explain the discrepancy of results obtained with the use of phospho-MEK antibody regarding the activation state of MEK in mitosis. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：675 / 680

页数：6

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