Solvatochromism of the green fluorescence protein chromophore and its derivatives

被引：209

作者：

Dong, Jian ^{[1
]}

Solntsev, Kyril M. ^{[1
]}

Tolbert, Laren M. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 37期

关键词：

D O I：

10.1021/ja063128a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The solvatochromic behavior of the green fluorescence protein (GFP) chromophore (p-hydroxybenzylideneimidazolone, p-HBDI) and its derivatives (p-methoxybenzylideneimidazolone, p-MeOBDI, and N-methyl-p-hydroxybenzylideneimidazolonium iodide, p-HBDIMe+) was studied using UV-vis-absorption spectroscopy in a wide array of solvents. The relative contribution of specific (polarity) vs nonspecific (hydrogen-bonding) solvation to the absorbance spectra was studied. On the basis of these data, we discuss the nature of the absorption peak of the protonated and deprotonated forms of the wild-type GFP. Copyright © 2006 American Chemical Society.

引用

页码：12038 / 12039

页数：2

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