Aspirin and salicylic acid do not inhibit methyl jasmonate-inducible expression of a gene for ornithine decarboxylase in tobacco BY-2 cells

被引：17

作者：

Imanishi, S ^{[1
]}

Nakakita, M ^{[1
]}

Yamashita, K ^{[1
]}

Furuta, A ^{[1
]}

Utsuno, K ^{[1
]}

Muramoto, N ^{[1
]}

Kojima, H ^{[1
]}

Nakamura, K ^{[1
]}

机构：

[1] Nagoya Univ, Biochem Lab, Dept Biol Mechanisms & Funct, Grad Sch Bioagr Sci,Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 01期

关键词：

jasmonate induction; salicylic acid; aspirin; ornithine decarboxylase gene of tobacco; tobacco cells;

D O I：

10.1271/bbb.64.125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Similar to the prostanoid-mediated inflammatory response in mammals, jasmonate-mediated wound response in plant leaves is inhibited by salicylic acid (SA) or acetylsalicylate (aspirin). In tobacco BY-2 cells, expression of the gene for ornithine decarboxylase (ODC) involved in putrescine synthesis is rapidly inducible by methyl jasmonate (MeJA), A nuclear gene for ODC isolated from tobacco, gNtODC-1, was an intron-less gene and MeJA induced the expression of a GUS fusion gene with the gNtODC-1 promoter in transformed tobacco cells. Although SA alone did not induce the expression, 0.2 to 20 mu M SA increased the MeJA-induced expression of the fusion gene to about two-fold. A similar increase was observed with aspirin but not with 3- or 4-hydroxybenzoic acids. SA at concentrations up to 200 mu M did not inhibit the MeJA-induction of mRNAs for the GUS fusion gene and the endogenous gene for ODC.

引用

页码：125 / 133

页数：9

共 34 条

[1] Quantification, correlations and manipulations of wound-induced changes in jasmonic acid and nicotine in Nicotiana sylvestris [J].