Biosynthesis of the enediyne antitumor antibiotic C-1027

被引：250

作者：

Liu, W

Christenson, SD

Standage, S

Shen, B ^{[1
]}

机构：

[1] Univ Wisconsin, Div Pharmaceut Sci, Madison, WI 53705 USA

[2] Univ Wisconsin, Dept Chem, Madison, WI 53705 USA

[3] Univ Calif Davis, Biochem & Mol Biol Grad Grp, Davis, CA 95616 USA

[4] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

来源：

SCIENCE | 2002年 / 297卷 / 5584期

关键词：

D O I：

10.1126/science.1072110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

C-1027 is a potent antitumor agent with a previously undescribed molecular architecture and mode of action. Cloning and characterization of the 85-kilobase C-1027 biosynthesis gene cluster from Streptomyces globisporus revealed (i) an iterative type I polyketide synthase that is distinct from any bacterial polyketide synthases known to date, (ii) a general polyketide pathway for the biosynthesis of both the 9- and 10-membered enediyne antibiotics, and (iii) a convergent biosynthetic strategy for the C-1027 chromophore from four building blocks. Manipulation of genes governing C-1027 biosynthesis allowed us to produce an enediyne compound in a predicted manner.

引用

页码：1170 / 1173

页数：5

共 32 条

[1] The calicheamicin gene cluster and its iterative type I enediyne PKS [J].