HIV-1 Virion Production from Single Inducible Proviruses following T-Cell Activation Ex Vivo

被引：21

作者：

Bui, John K. ^{[1
]}

Mellors, John W. ^{[1
]}

Cillo, Anthony R. ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Med, Dept Med, Div Infect Dis, Pittsburgh, PA 15213 USA

来源：

JOURNAL OF VIROLOGY | 2016年 / 90卷 / 03期

关键词：

SUPPRESSIVE ANTIRETROVIRAL THERAPY; LATENT RESERVOIR; IN-VIVO; CURE; QUANTIFICATION; PERSISTENCE; EXPRESSION; INFECTION; SUBSETS; VIREMIA;

D O I：

10.1128/JVI.02520-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Quantifying induced virion production from single proviruses is important for assessing the effects of HIV-1 latency reversal agents. Limiting dilution ex vivo cultures of resting CD4(+) T cells from 14 HIV-positive volunteers revealed that virion production after T-cell activation from individual proviruses varies by 10,000-fold to 100,000-fold. High-producing proviruses were associated with increases in cell-associated HIV-1 DNA levels, suggesting that reactivated proviruses proliferate. Single-cell analyses are needed to investigate differences in proviral expansion and virus production following latency reversal.

引用

页码：1673 / 1676

页数：4

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