Inhibitory activity of brown algal phlorotannins against hyaluronidase

被引：159

作者：

Shibata, T ^{[1
]}

Fujimoto, K ^{[1
]}

Nagayama, K ^{[1
]}

Yamaguchi, K ^{[1
]}

Nakamura, T ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Agr, Dept Biosci & Biotechnol, Lab Marine Resource Chem,Higashi Ku, Fukuoka 8128581, Japan

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2002年 / 37卷 / 06期

关键词：

Ecklonia kurome; Eisenia bicyclis; enzyme inhibition; polyphenols;

D O I：

10.1046/j.1365-2621.2002.00603.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The inhibitory effect of brown algal phlorotannins on hyaluronidase was evaluated by an in vitro assay. Crude phlorotannins from the brown algae Eisenia bicyclis and Ecklonia kurome had a stronger inhibitory effect than well-known inhibitors such as catechins and sodium cromoglycate. IC50 values of the following six phlorotannins: phloroglucinol, an unknown tetramer, eckol (a trimer), phlorofucofuroeckol A (a pentamer), dieckol and 8,8'-bieckol (hexamers), were 280, 650, >800, 140, 120 and 40 muM, respectively. The IC50 of catechin, epigallocatechin gallate and sodium cromoglycate was 620, 190 and 270 muM, respectively. 8,8'-Bieckol, the strongest HAase inhibitor in this study, acted as a competitive inhibitor with an inhibition constant (K (i) ) of 35 muM. Acetylation of the phlorotannins markedly decreased their inhibitory potency.

引用

页码：703 / 709

页数：7

共 29 条

[1] A MACROCIRCULAR ELLAGITANNIN, OENOTHEIN-B, SUPPRESSES MOUSE MAMMARY-TUMOR GENE-EXPRESSION VIA INHIBITION OF POLY(ADP-RIBOSE) GLYCOHYDROLASE [J].