Crystallization of engineered Thermus flavus 5S rRNA under earth and microgravity conditions

被引：18

作者：

Lorenz, S

Perbandt, M

Lippmann, C

Moore, K

DeLucas, LJ

Betzel, C

Erdmann, VA

机构：

[1] Free Univ Berlin, Inst Biochem, D-14195 Berlin, Germany

[2] Univ Hamburg, Krankenhaus Eppendorf, Inst Biomed Biochem & Mol Biol, Arbeitsgrp Makromol Strukturanal, D-22603 Hamburg, Germany

[3] Univ Alabama Birmingham, Ctr Macromol Crystallog, Birmingham, AL 35294 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2000年 / 56卷

关键词：

D O I：

10.1107/S0907444900001736

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Thermus flavus 5S rRNA with a molecular weight of about 40 kDa was modified at the 5' and 3' ends. Crystals were obtained under earth and microgravity conditions. The best crystals were obtained during NASA space mission STS 94. For the first time, it was possible to collect a complete data set from 5S rRNA crystals to 7.8 Angstrom resolution and to assign the space group as R32. with unit-cell parameters a = b = 110.3, c = 387.6 Angstrom, alpha = beta = 90, gamma = 120 degrees.

引用

收藏

页码：498 / 500

页数：3

相关论文

共 17 条

[1] CRYSTAL-STRUCTURE OF DOMAIN-A OF THERMUS-FLAVUS 5S RIBOSOMAL-RNA AND THE CONTRIBUTION OF WATER-MOLECULES TO ITS STRUCTURE [J].

BETZEL, C ;

LORENZ, S ;

FURSTE, JP ;

BALD, R ;

ZHANG, M ;

SCHNEIDER, TR ;

WILSON, KS ;

ERDMANN, VA .

FEBS LETTERS, 1994, 351 (02) :159-164

[2] Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain [J].

Correll, CC ;

Freeborn, B ;

Moore, PB ;

Steitz, TA .

CELL, 1997, 91 (05) :705-712

[3] TOTAL RECONSTITUTION AND ASSEMBLY OF 50 S-SUBUNITS FROM ESCHERICHIA-COLI RIBOSOMES INVITRO [J].

DOHME, F ;

NIERHAUS, KH .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 107 (04) :585-&

[4] STRUCTURE AND FUNCTION OF BACTERIAL RIBOSOMES .13. ROLE OF 5S RNA IN FUNCTIONS OF 50S RIBOSOMAL SUBUNITS [J].

ERDMANN, VA ;

FAHNESTOCK, S ;

HIGO, K ;

NOMURA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (12) :2932-+

[5] INVITRO INCORPORATION OF EUBACTERIAL, ARCHAEBACTERIAL AND EUKARYOTIC 5S RIBOSOMAL-RNAS INTO LARGE RIBOSOMAL-SUBUNITS OF BACILLUS-STEAROTHERMOPHILUS [J].

HARTMANN, RK ;

VOGEL, DW ;

WALKER, RT ;

ERDMANN, VA .

NUCLEIC ACIDS RESEARCH, 1988, 16 (08) :3511-3524

[6]

HORNE JR, 1972, MOL GEN GENET, V119, P337, DOI 10.1007/BF00272091

[7] CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF 5-S RIBOSOMAL-RNA FROM THE THERMOPHILIC BACTERIUM THERMUS-FLAVUS [J].

LORENZ, S ;

BETZEL, C ;

RADERSCHALL, E ;

DAUTER, Z ;

WILSON, KS ;

ERDMANN, VA .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 219 (03) :399-402

[8] CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF THE CHEMICALLY SYNTHESIZED DOMAIN-A OF THERMUS-FLAVUS 5S RIBOSOMAL-RNA - AN RNA DODECAMER DOUBLE HELIX [J].

LORENZ, S ;

FURSTE, JP ;

BALD, R ;

ZHANG, M ;

RADERSCHALL, E ;

BETZEL, C ;

DAUTER, Z ;

WILSON, KS ;

ERDMANN, VA .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1993, 49 :418-420

[9] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[10] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDY OF 5-S RIBOSOMAL-RNA FROM THERMUS-THERMOPHILUS HB8 [J].

MORIKAWA, K ;

KAWAKAMI, M ;

TAKEMURA, S .

FEBS LETTERS, 1982, 145 (02) :194-196