CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF THE CHEMICALLY SYNTHESIZED DOMAIN-A OF THERMUS-FLAVUS 5S RIBOSOMAL-RNA - AN RNA DODECAMER DOUBLE HELIX

被引：13

作者：

LORENZ, S

FURSTE, JP

BALD, R

ZHANG, M

RADERSCHALL, E

BETZEL, C

DAUTER, Z

WILSON, KS

ERDMANN, VA

机构：

[1] FREE UNIV BERLIN, INST BIOCHIM, THIELALLEE 63, W-1000 BERLIN 33, GERMANY

[2] DESY, EMBL, W-2000 HAMBURG 52, GERMANY

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1993年 / 49卷

关键词：

D O I：

10.1107/S0907444993001520

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of domain A of Thermus flavus 5S rRNA have been obtained. The space group was found to be P4(3) With unit-cell dimensions a = b = 30. 10 and c = 86.80 angstrom. Data to 2.3 angstrom have been recorded and solution of the structure is currently underway by means of molecular-replacement techniques.

引用

收藏

页码：418 / 420

页数：3

相关论文

共 12 条

[1]

BALD R, 1992, STRUCTURAL TOOLS ANA, P449

[2] CRYSTALLOGRAPHIC REFINEMENT BY SIMULATED ANNEALING - APPLICATION TO CRAMBIN [J].

BRUNGER, AT ;

KARPLUS, M ;

PETSKO, GA .

ACTA CRYSTALLOGRAPHICA SECTION A, 1989, 45 :50-61

[3] CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX - [U(UA)6A]2 [J].

DOCKBREGEON, AC ;

CHEVRIER, B ;

PODJARNY, A ;

JOHNSON, J ;

DEBEAR, JS ;

GOUGH, GR ;

GILHAM, PT ;

MORAS, D .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (03) :459-474

[4] STRUCTURE AND FUNCTION OF BACTERIAL RIBOSOMES .13. ROLE OF 5S RNA IN FUNCTIONS OF 50S RIBOSOMAL SUBUNITS [J].

ERDMANN, VA ;

FAHNESTOCK, S ;

HIGO, K ;

NOMURA, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (12) :2932-+

[5]

ERDMANN VA, 1993, IN PRESS TRANSLATION

[6] INVITRO INCORPORATION OF EUBACTERIAL, ARCHAEBACTERIAL AND EUKARYOTIC 5S RIBOSOMAL-RNAS INTO LARGE RIBOSOMAL-SUBUNITS OF BACILLUS-STEAROTHERMOPHILUS [J].

HARTMANN, RK ;

VOGEL, DW ;

WALKER, RT ;

ERDMANN, VA .

NUCLEIC ACIDS RESEARCH, 1988, 16 (08) :3511-3524

[7] EVALUATION OF SINGLE-CRYSTAL X-RAY-DIFFRACTION DATA FROM A POSITION-SENSITIVE DETECTOR [J].

KABSCH, W .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1988, 21 (06) :916-924

[8] CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF 5-S RIBOSOMAL-RNA FROM THE THERMOPHILIC BACTERIUM THERMUS-FLAVUS [J].

LORENZ, S ;

BETZEL, C ;

RADERSCHALL, E ;

DAUTER, Z ;

WILSON, KS ;

ERDMANN, VA .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 219 (03) :399-402

[9] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[10]

NAVAZA J, 1992, P DARESBURY STUDY WE