Flux control of the bacterial phosphoenolpyruvate:glucose phosphotransferase system and the effect of diffusion

被引：7

作者：

Francke, C

Westerhoff, HV

Blom, JG

Peletier, MA

机构：

[1] Free Univ Amsterdam, Dept Mol Cell Physiol, Bioctr Amsterdam, NL-1081 HV Amsterdam, Netherlands

[2] Univ Amsterdam, SILS, Bioctr Amsterdam, NL-1012 WX Amsterdam, Netherlands

[3] Stellenbosch Inst Adv Study, Stellenbosch, South Africa

[4] CWI, Ctr Math & Informat, NL-1090 GB Amsterdam, Netherlands

[5] Eindhoven Univ Technol, NL-5600 MB Eindhoven, Netherlands

来源：

MOLECULAR BIOLOGY REPORTS | 2002年 / 29卷 / 1-2期

关键词：

D O I：

10.1023/A:1020389915464

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed the role of diffusion and cell size on the flux control properties of the glucose-PTS of Escherichia coli, in silicon cells under various metabolic conditions. To our surprise, the influence of the concentration of phosphoryl-donor PEP on the distribution of control was small. We found for cells of bacterial size that PTS-flux control was mainly located in processes taking place in the membrane and that diffusion hardly controlled the flux (< 2.8 %). Enlargement of the cells shifted the control from membrane to cytoplasm and from process rates to diffusion rates, the latter now having a total control of about 38 %. In the presence of glucose, nearly all diffusion flux control resided in the component that links the cytoplasmic processes to those in the membrane.

引用

页码：21 / 26

页数：6