Structural Characteristics and Antioxidant Activities of Oligosaccharides from Longan Fruit Pericarp

被引：42

作者：

Jiang, Guoxiang ^{[1
]}

Jiang, Yueming ^{[1
]}

Yang, Bao ^{[1
]}

Yu, Chunyan ^{[1
]}

Tsao, Rong ^{[2
]}

Zhang, Haiyan ^{[1
]}

Chen, Feng ^{[3
]}

机构：

[1] Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China

[2] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada

[3] Clemson Univ, Dept Food Sci & Human Nutr, Clemson, SC 29634 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 19期

基金：

中国国家自然科学基金;

关键词：

Longan; oligosaccharide; ultrasonic assisted extraction; response surface methodology; antioxidant activity; RESPONSE-SURFACE METHODOLOGY; ULTRASOUND-ASSISTED EXTRACTION; RADICAL SCAVENGING ACTIVITY; POLYPHENOLIC COMPOUNDS; OPTIMIZATION; FOOD; ACID; POLYSACCHARIDE; IDENTIFICATION; DERIVATIVES;

D O I：

10.1021/jf902534v

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

Ultrasonic assisted extraction was employed to extract oligosaccharides from longan fruit pericarp (OLFP). A Box-Behnken design was applied to investigate the effects of ultrasonic temperature (30-70 degrees C), power (120-300 W), and time (10-50 min) on OLFP recovery. The model showed a good agreement with the experimental results on the basis of R-2 of 0.9655 and P-value <0.05. From response surface plots, ultrasonic power, time, and temperature exhibited independent and interactive effects on OLFP recovery. The optimal conditions to obtain the highest OLFP recovery were determined to be 13 min, 121 W, and 65 degrees C. Gas chromatography analysis indicated that purified OLFP comprised Gal (71.5%), GIc (24.6%), and GaIA (3.9%). The analysis of glycosidic linkages showed that the backbone consisted of -> 3)-Gal-(1 ->, -> 6)-Gal-(1 ->, GIc-(1 -> and -> 3)-GaIA-(l -> with a molar proportion of 13:5:6:1. Furthermore, the 1,1-diphenyl-2-picryldydrazyl (DPPH) and superoxide anion radical scavenging assays showed that OLFP exhibited strong antioxidant activities in a dose-dependent manner.

引用

页码：9293 / 9298

页数：6

共 35 条

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