Silver-Ion-Mediated DNAzyme Switch for the Ultrasensitive and Selective Colorimetric Detection of Aqueous Ag+ and Cysteine

被引：239

作者：

Li, Tao ^{[1
,2
]}

Shi, Lili ^{[3
]}

Wang, Erkang ^{[1
,2
]}

Dong, Shaojun ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] NE Normal Univ, Dept Chem, Changchun 130024, Jilin, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2009年 / 15卷 / 14期

基金：

中国国家自然科学基金;

关键词：

DNA structures; enzyme catalysis; G-quadruplexes; nucleobases; sensors; FUNCTIONALIZED GOLD NANOPARTICLES; BASE-PAIR; G-QUADRUPLEX; CATALYTIC BEACONS; DNA DUPLEXES; MERCURY(II); HG2+; LABEL; THROMBIN; COMPLEX;

D O I：

10.1002/chem.200900056

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly selective method for ultrasensitive colorimetric Ag+ detection that utilizes a G-quadruplex-based DNAzyme was introduced. This process enables the selective detention of aqueous Ag+ in concentrations as low as 2.5 nm in a facile colorimetric way. The DNAzyme switch, in an open state, can be closed again by addition of Cys because of its ability to remove Ag+ from the C-Ag+-C base pairs. The experiment performed reveals high sensitivity for the analysis of Cys by using the DNAzyme switch. The sensing applications of this DNAzyme switch may be extended by substituting the C-C pairs with other native or artificial bases that specifically bind other metal ions.

引用

页码：3347 / 3350

页数：4

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