Silver-Ion-Mediated DNAzyme Switch for the Ultrasensitive and Selective Colorimetric Detection of Aqueous Ag+ and Cysteine

被引：239

作者：

Li, Tao ^{[1
,2
]}

Shi, Lili ^{[3
]}

Wang, Erkang ^{[1
,2
]}

Dong, Shaojun ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] NE Normal Univ, Dept Chem, Changchun 130024, Jilin, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2009年 / 15卷 / 14期

基金：

中国国家自然科学基金;

关键词：

DNA structures; enzyme catalysis; G-quadruplexes; nucleobases; sensors; FUNCTIONALIZED GOLD NANOPARTICLES; BASE-PAIR; G-QUADRUPLEX; CATALYTIC BEACONS; DNA DUPLEXES; MERCURY(II); HG2+; LABEL; THROMBIN; COMPLEX;

D O I：

10.1002/chem.200900056

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly selective method for ultrasensitive colorimetric Ag+ detection that utilizes a G-quadruplex-based DNAzyme was introduced. This process enables the selective detention of aqueous Ag+ in concentrations as low as 2.5 nm in a facile colorimetric way. The DNAzyme switch, in an open state, can be closed again by addition of Cys because of its ability to remove Ag+ from the C-Ag+-C base pairs. The experiment performed reveals high sensitivity for the analysis of Cys by using the DNAzyme switch. The sensing applications of this DNAzyme switch may be extended by substituting the C-C pairs with other native or artificial bases that specifically bind other metal ions.

引用

页码：3347 / 3350

页数：4

共 39 条

[21] Multifunctional G-Quadruplex Aptamers and Their Application to Protein Detection [J].

Li, Tao ;

Shi, Lili ;

Wang, Erkang ;

Dong, Shaojun .

CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (04) :1036-1042

[22] Detection of mercury(II) based on Hg2+-DNA complexes inducing the aggregation of gold nanoparticles [J].

Liu, Chi-Wei ;

Hsieh, Yi-Ting ;

Huang, Chih-Ching ;

Lin, Zong-Hong ;

Chang, Huan-Tsung .

CHEMICAL COMMUNICATIONS, 2008, (19) :2242-2244

[23] Rational design of "Turn-On" allosteric DNAzyme catalytic beacons for aqueous mercury ions with ultrahigh sensitivity and selectivity [J].

Liu, Juewen ;

Lu, Yi .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (40) :7587-7590

[24] Optical detection of mercury(II) in aqueous solutions by using conjugated polymers and label-free oligonucleotides [J].

Liu, Xingfen ;

Tang, Yanli ;

Wang, Lihua ;

Zhang, Juan ;

Song, Shiping ;

Fan, Chunhai ;

Wang, Shu .

ADVANCED MATERIALS, 2007, 19 (11) :1471-+

[25] MercuryII-mediated formation of thymine-HgII-thymine base pairs in DNA duplexes [J].

Miyake, Y ;

Togashi, H ;

Tashiro, M ;

Yamaguchi, H ;

Oda, S ;

Kudo, M ;

Tanaka, Y ;

Kondo, Y ;

Sawa, R ;

Fujimoto, T ;

Machinami, T ;

Ono, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (07) :2172-2173

[26] Specific interactions between silver(I) ions and cytosine-cytosine pairs in DNA duplexes [J].

Ono, Akira ;

Cao, Shiqi ;

Togashi, Humika ;

Tashiro, Mitsuru ;

Fujimoto, Takashi ;

Machinami, Tomoya ;

Oda, Shuji ;

Miyake, Yoko ;

Okamoto, Itaru ;

Tanaka, Yoshiyuki .

CHEMICAL COMMUNICATIONS, 2008, (39) :4825-4827

[27] An artificial base pair, mediated by hydrogen bonding and metal-ion binding [J].

Polonius, Fabian-Alexander ;

Mueller, Jens .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (29) :5602-5604

[28] DNA enzymes [J].

Sen, D ;

Geyer, CR .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (06) :680-687

[29] A purine-like nickel(II) base pair for DNA [J].

Switzer, C ;

Sinha, S ;

Kim, PH ;

Heuberger, BD .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (10) :1529-1532

[30] Formation of silver(I)-mediated DNA duplex and triplex through an alternative base pair of pyridine nucleobases [J].

Tanaka, K ;

Yamada, Y ;

Shionoya, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (30) :8802-8803

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