Effect of phytohormone biosynthesis genes (ipt and iaaM plus iaaH) on the sexual reproduction of transgenic tobacco plants

被引:2
作者
Bavrina, TV
Milyaeva, EL
Machácková, I
Pustovoitova, TN
Gurko, NA
Kasumova, IV
Zhdanova, NE
机构
[1] Russian Acad Sci, KA Timiryazev Plant Physiol Inst, Moscow 127276, Russia
[2] Acad Sci Czech Republ, Inst Expt Bot, Prague, Czech Republic
关键词
Nicotiana silvestris; phytohormones; transgenic plants; pollen; pollination; seeds; ipt-gene; (iaaM plus iaaH)-genes;
D O I
10.1023/A:1016355824539
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transformation of Nicotiana silvestris Spegar et Comes plants by the ipt or iaaM + iaaH genes changed the hormonal status of plant reproductive organs. The total content of cytokinins and ABA increased, whereas IAA content in the pistils and anthers of the ipt-plants did not change. Reduced fertility of the ipt plants correlated with an elevated cytokinin and ABA contents of their reproductive organs. Pollen tubes of these plants showed defective growth in pistils in situ, and ovaries manifested a low metabolic activity. The transgenic (iaaM + iaaH)-plants were characterized by an elevated IAA content and reduced ABA content, whereas the total content of cytokinins did not change. The fertility of these -plants did not differ from that in the wild type.
引用
收藏
页码:484 / 491
页数:8
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