Atomic force microscopy characterization of an electrochemical DNA-biosensor

被引：40

作者：

Chiorcea, AM ^{[1
]}

Brett, AMO ^{[1
]}

机构：

[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

来源：

BIOELECTROCHEMISTRY | 2004年 / 63卷 / 1-2期

关键词：

DNA-biosensor; dsDNA; HOPG; AFM; atomic force microscopy;

D O I：

10.1016/j.bioelechem.2003.09.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrode surface characteristics represent an important aspect on the construction of sensitive DNA electrochemical biosensors for rapid detection of DNA interaction and damage. Two different immobilization procedures of double-stranded DNA (dsDNA) at the surface of a HOPG electrode were evaluated by MAC mode AFM performed in air. A thin dsDNA adsorbed film forming a network structure with holes exposing the electrode surface and a thick dsDNA film completely covering the electrode surface, presenting a much rougher structure, were investigated. The DNA surface characteristics and structure are discussed with respect to the degree of surface coverage. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：229 / 232

页数：4

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