Atomic force microscopy characterization of an electrochemical DNA-biosensor

被引:40
作者
Chiorcea, AM [1 ]
Brett, AMO [1 ]
机构
[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal
关键词
DNA-biosensor; dsDNA; HOPG; AFM; atomic force microscopy;
D O I
10.1016/j.bioelechem.2003.09.029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electrode surface characteristics represent an important aspect on the construction of sensitive DNA electrochemical biosensors for rapid detection of DNA interaction and damage. Two different immobilization procedures of double-stranded DNA (dsDNA) at the surface of a HOPG electrode were evaluated by MAC mode AFM performed in air. A thin dsDNA adsorbed film forming a network structure with holes exposing the electrode surface and a thick dsDNA film completely covering the electrode surface, presenting a much rougher structure, were investigated. The DNA surface characteristics and structure are discussed with respect to the degree of surface coverage. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:229 / 232
页数:4
相关论文
共 7 条
[1]   Denaturation and association of DNA sequences by certain polypropylene surfaces [J].
Belotserkovskii, BP ;
Johnston, BH .
ANALYTICAL BIOCHEMISTRY, 1997, 251 (02) :251-262
[2]   Voltammetric behaviour of mitoxantrone at a DNA-biosensor [J].
Brett, AMO ;
Macedo, TRA ;
Raimundo, D ;
Marques, MH ;
Serrano, SHP .
BIOSENSORS & BIOELECTRONICS, 1998, 13 (7-8) :861-867
[3]   A magnetically driven oscillating probe microscope for operation in liquids [J].
Han, WH ;
Lindsay, SM ;
Jing, TW .
APPLIED PHYSICS LETTERS, 1996, 69 (26) :4111-4113
[4]   DNA electrochemical biosensors [J].
Mascini, M ;
Palchetti, I ;
Marrazza, G .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2001, 369 (01) :15-22
[5]  
Oliveira-Brett A.M., 1999, COMPREHENSIVE CHEM K, V37, P91
[6]   Electrochemical detection of in situ adriamycin oxidative damage to DNA [J].
Oliveira-Brett, AM ;
Vivan, M ;
Fernandes, IR ;
Piedade, JAP .
TALANTA, 2002, 56 (05) :959-970
[7]   Past, present and future of nucleic acids electrochemistry [J].
Palecek, E .
TALANTA, 2002, 56 (05) :809-819