Reversible Conformational Changes of PsbO Protein Detected by Terahertz Time-Domain Spectroscopy

被引：1

作者：

Chen Hua ^{[1
]}

Chen Gui-Ying ^{[2
]}

Li Shu-Qin ^{[2
]}

Wang Li ^{[3
]}

机构：

[1] Southeast Univ, Dept Phys, Nanjing 211189, Peoples R China

[2] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Photosynth Res Ctr, Beijing 100093, Peoples R China

[3] Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China

来源：

CHINESE PHYSICS LETTERS | 2009年 / 26卷 / 08期

关键词：

MANGANESE-STABILIZING PROTEIN; PHOTOSYSTEM-II;

D O I：

暂无

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We used a terahertz time-domain spectroscope (THz-TDS) to detect the reversible conformational changes of PsbO protein induced by N-bromosuccinimide and Guanidine Hydrochloride. The veracity and sensitivity are confirmed by the fluorescence emission spectra. The results demonstrate that THz-TDS has both advantages and disadvantages in monitoring the denaturation process of proteins, which is important in applying THz-TDS technique to studying biomolecules.

引用

页数：3

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