Reversible Conformational Changes of PsbO Protein Detected by Terahertz Time-Domain Spectroscopy

被引:1
作者
Chen Hua [1 ]
Chen Gui-Ying [2 ]
Li Shu-Qin [2 ]
Wang Li [3 ]
机构
[1] Southeast Univ, Dept Phys, Nanjing 211189, Peoples R China
[2] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
[3] Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China
关键词
MANGANESE-STABILIZING PROTEIN; PHOTOSYSTEM-II;
D O I
暂无
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
We used a terahertz time-domain spectroscope (THz-TDS) to detect the reversible conformational changes of PsbO protein induced by N-bromosuccinimide and Guanidine Hydrochloride. The veracity and sensitivity are confirmed by the fluorescence emission spectra. The results demonstrate that THz-TDS has both advantages and disadvantages in monitoring the denaturation process of proteins, which is important in applying THz-TDS technique to studying biomolecules.
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页数:3
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