共 16 条
Reversible Conformational Changes of PsbO Protein Detected by Terahertz Time-Domain Spectroscopy
被引:1
作者:
Chen Hua
[1
]
Chen Gui-Ying
[2
]
Li Shu-Qin
[2
]
Wang Li
[3
]
机构:
[1] Southeast Univ, Dept Phys, Nanjing 211189, Peoples R China
[2] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
[3] Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China
关键词:
MANGANESE-STABILIZING PROTEIN;
PHOTOSYSTEM-II;
D O I:
暂无
中图分类号:
O4 [物理学];
学科分类号:
0702 ;
摘要:
We used a terahertz time-domain spectroscope (THz-TDS) to detect the reversible conformational changes of PsbO protein induced by N-bromosuccinimide and Guanidine Hydrochloride. The veracity and sensitivity are confirmed by the fluorescence emission spectra. The results demonstrate that THz-TDS has both advantages and disadvantages in monitoring the denaturation process of proteins, which is important in applying THz-TDS technique to studying biomolecules.
引用
收藏
页数:3
相关论文